Pathways Animal Hospital is a full-service canine reproduction practice at 6543 Main St, Williamsville, minutes from Buffalo. We provide same-day in-house progesterone testing, vaginal, transcervical and surgical insemination, semen collection, evaluation, freezing and long-term storage as a Certified Zoetis Freezing Center, pregnancy ultrasound and puppy counts, planned cesarean sections, neonatal care, and OFA, PennHIP and SV/WUSV radiographs. Breeders do not need a referral. We do ask for a current annual examination with us, a breeding soundness examination for each cycle you breed on, current vaccines and parasite screening, and health testing appropriate to the breed. Call 716.936.8387 or email repro@pahwny.com.
- Same-day progesterone, run in-house by ELFA
- >90% success rate with transcervical insemination
- 25+ years — litters produced from semen frozen that long
- M/W/F 8–5 · T/Th 8–7
Planning to breed this year? Talk to us before her season starts.
Why breeders drive past their own vet to get here
Most general practices can draw a progesterone sample. Far fewer can run it the same morning, interpret it against the cycle in front of them, and have a transcervical insemination set up before the window closes. Reproductive work is a timing discipline, and timing is where it is usually lost.
We run progesterone in-house rather than sending it out, which is the single biggest practical difference for a breeder. A sample sent to an outside laboratory comes back tomorrow. Ours comes back today, and the decision gets made today — which is what matters when you are working with chilled or frozen semen and the fertile window is measured in hours rather than days.

No referral needed. Ask us what your program still needs.
What we do for breeding programs
Ovulation timing
Same-day in-house progesterone by ELFA, plus vaginal cytology, so the fertile window is pinpointed rather than guessed.
Artificial insemination
Vaginal, transcervical (TCI) and surgical. TCI needs no anesthesia and runs above ninety percent in our hands.
Frozen semen
Collection, evaluation, freezing and long-term storage as a Certified Zoetis Freezing Center.
Chilled semen shipping
Collection, extension and shipping out, plus receiving and evaluating shipments coming in.
Brucellosis testing
Pre-breeding screening for both dams and studs, which no responsible program should skip.
Pregnancy diagnosis
Ultrasound from around 28 days for confirmation and heartbeats; radiographs late for an accurate puppy count.
Planned C-sections
Scheduled from ovulation timing, with puppies delivered into a warm, oxygen-rich environment by trained staff.
Neonatal & pediatric care
Tube feeding, plasma administration, antibiotics and early puppy work when a litter needs support.
Health clearances
OFA, PennHIP and SV/WUSV radiographs, plus thyroid, cardiac, patella, dentition, shoulder, spine and trachea.
Stud dog work-ups
Semen evaluation and fertility assessment before a stud is advertised, not after a breeding fails.
Not sure which of these you need? Tell us the cycle and we will talk it through.
Ovulation timing, and why same-day matters
Progesterone testing usually begins early in the heat cycle and repeats every one to two days as values rise, so ovulation is pinpointed rather than estimated. Vaginal cytology runs alongside it. The aim is a date, not a range.
With fresh semen and a proven pair you have some margin for error. With chilled semen shipped overnight, or with frozen semen that has a fertile life measured in hours once thawed, you have almost none. That is the case where in-house testing stops being a convenience and starts being the difference between a litter and an empty season.
Insemination: vaginal, transcervical and surgical
Transcervical insemination (TCI) places semen directly into the uterus through the cervix under endoscopic guidance, with the bitch awake and no anesthesia required. It is well tolerated, can be repeated within a cycle, and in our hands succeeds more than ninety percent of the time. For chilled semen, and for bitches who have not conceived from vaginal insemination, it is usually the right call.
Surgical insemination remains appropriate in specific cases, particularly with limited or poor-quality frozen semen where every straw counts. We will tell you honestly which technique your situation calls for rather than defaulting to the most involved one.
Frozen semen: a stud dog outlives his career
As a Certified Zoetis Freezing Center we collect, evaluate, freeze and store canine semen long-term. Properly frozen and stored, it stays viable for decades — Dr. Gray has produced litters from semen preserved for more than twenty-five years.
For a breeding program that is not a novelty, it is insurance. A stud who is collected while he is sound and in his prime remains available to the breed long after he has retired, and long after he is gone. Breeders travel here from across Pennsylvania and Southern Ontario specifically for that work.
Ask about collection, storage terms and shipping straws to another practice.
Pregnancy, puppy counts and planned cesareans
Ultrasound at around 28 days after ovulation confirms pregnancy and shows fetal heartbeats with an approximate count. An accurate number needs a radiograph about a week before the due date, once the fetal skeletons have mineralized — and that number is a safety check during whelping, because it is how you know the litter is actually finished.
A planned cesarean, timed from ovulation and progesterone values, is a very different event from an emergency section at the end of a long, failing labour. Our dams recover without external sutures, puppies are delivered into a warm, oxygen-rich environment by trained staff, and families are typically home within one to two hours.

Health clearances and breeder radiographs
We take OFA hip and elbow films regularly, and those can often be positioned without sedation. PennHIP and SV/WUSV submissions do require sedation, because both depend on positioning that cannot be achieved safely or accurately in an awake dog. Correct positioning is everything either way: a poorly positioned hip can cost a dog a grade it did not deserve.
Beyond hips and elbows we handle thyroid, basic cardiac, patella and dentition clearances, along with shoulder, spine and trachea radiographs. The only clearances we do not offer are those requiring a specialist — eye certification and advanced cardiac — and we will point you to the right referral for those.
Planning clearances for a litter? We can usually do several in one visit.
Where our breeder clients come from
We are in Williamsville, immediately east of Buffalo and just off the I-90 and I-290. Breeders travel to us from across Western New York and beyond — for much of the region we are considerably closer than the nearest university teaching hospital.
Erie County — under 30 minutes
Same-day progesterone with no planning required. Most of our breeder clients are here.
- Buffalo
- Williamsville
- Amherst
- Clarence
- Cheektowaga
- Lancaster
- Depew
- Tonawanda
- North Tonawanda
- Kenmore
- West Seneca
- Orchard Park
- Hamburg
- East Aurora
- Grand Island
- Elma
- Alden
- Eden
- Boston
- Springville
- Akron
- Lackawanna
- Blasdell
- Snyder
- Getzville
- Sloan
Niagara, Genesee & Wyoming Counties — 30 to 60 minutes
Comfortable for a same-day draw and an insemination in the same trip.
- Niagara Falls
- Lockport
- Lewiston
- Youngstown
- Wilson
- Newfane
- Middleport
- Medina
- Albion
- Batavia
- Le Roy
- Attica
- Warsaw
- Perry
- Arcade
- Holland
- Chaffee
- Sanborn
- Barker
- Wolcottsville
Chautauqua, Cattaraugus, Allegany & Orleans — 1 to 2 hours
Breeders here often plan a single trip around an ovulation date we have already predicted.
- Jamestown
- Dunkirk
- Fredonia
- Westfield
- Mayville
- Silver Creek
- Gowanda
- Olean
- Salamanca
- Ellicottville
- Little Valley
- Franklinville
- Wellsville
- Cuba
- Angelica
- Belmont
- Portville
Rochester, the Finger Lakes & the Southern Tier — 1½ to 3 hours
Regular clients drive in for TCI, surgical AI and planned cesareans rather than travel to Ithaca.
- Rochester
- Greece
- Henrietta
- Webster
- Pittsford
- Brockport
- Canandaigua
- Geneva
- Newark
- Palmyra
- Victor
- Hornell
- Bath
- Corning
- Elmira
- Dansville
- Geneseo
- Avon
- Mount Morris
Northwest Pennsylvania & the Ohio border — 1½ to 3 hours
Frozen semen storage and planned C-sections are the usual reasons for the drive.
- Erie, PA
- North East, PA
- Meadville, PA
- Corry, PA
- Warren, PA
- Bradford, PA
- Titusville, PA
- Sharon, PA
- Ashtabula, OH
- Conneaut, OH
Southern Ontario — via the Peace Bridge or Rainbow Bridge
Canadian breeders cross for frozen semen work and ovulation timing. Bring current import paperwork.
- Fort Erie, ON
- Niagara Falls, ON
- St. Catharines, ON
- Welland, ON
- Port Colborne, ON
- Hamilton, ON
- Burlington, ON
- Dunnville, ON
Not on the list? Distance is rarely the deciding factor — plenty of our clients plan a single trip around a date we have already predicted for them. Call 716.936.8387 and we will tell you honestly whether the drive is worth it for what you need.
We will tell you honestly whether the drive makes sense for your situation.
Common questions from breeders
Where can I find a canine reproduction veterinarian near Buffalo, NY?
Pathways Animal Hospital, at 6543 Main St in Williamsville, is a full-service canine reproduction practice serving breeders across Western New York. We handle ovulation timing with same-day in-house progesterone, vaginal, transcervical and surgical insemination, semen collection, evaluation, freezing and long-term storage as a Certified Zoetis Freezing Center, pregnancy ultrasound and puppy counts, and planned cesarean sections. Call 716.936.8387.
What do you require before you will time a cycle?
No referral is needed, but we do ask for a few things. Every breeding dog needs a current annual examination with us, which is what allows us to treat a last-minute progesterone request as urgent rather than as a new-client enquiry. We also require a breeding soundness examination for each cycle you intend to breed on — not once a year, but each time, because a bitch’s fitness to carry a litter changes between seasons. Vaccines and parasite screening should be current, and we expect health testing appropriate to the breed. Full details are on our progesterone testing page.
How quickly can I get progesterone results?
Same day. We run progesterone in-house by ELFA rather than sending it to an outside laboratory, so a morning draw gives you a number the same day and a decision the same day. That matters most with chilled or frozen semen, where the fertile window is narrow and a day lost is a breeding lost.
How often does progesterone need to be tested to time a breeding?
Testing usually starts early in the heat cycle and repeats every one to two days as values rise, so ovulation is pinpointed rather than estimated. The exact schedule depends on the bitch and on what semen you are using.
What is transcervical insemination and how successful is it?
TCI places semen directly into the uterus through the cervix using endoscopic guidance, with the bitch awake and no anesthesia required. It is well tolerated, can be repeated, and in our hands has a success rate greater than ninety percent. It is often the preferred technique for chilled semen and for bitches where vaginal insemination has not produced a litter.
How long can frozen dog semen be stored and still produce a litter?
Properly frozen and stored canine semen remains viable for decades. Dr. Gray has produced litters from semen preserved for more than twenty-five years. As a Certified Zoetis Freezing Center we handle collection, evaluation, freezing and long-term storage, which keeps a stud dog available long after his breeding career ends.
When can pregnancy be confirmed, and when do you count puppies?
Ultrasound at around 28 days after ovulation confirms pregnancy and shows fetal heartbeats along with an approximate count. An accurate number requires a radiograph about a week before the due date, once the fetal skeletons have mineralized. That count is an important safety check during whelping, because it tells you when the litter is actually finished.
Why choose a planned C-section instead of waiting for labour?
A planned cesarean is scheduled from ovulation timing and progesterone values, so surgery happens on a healthy dam before a long or obstructed labour compromises her or the puppies. It is particularly recommended for brachycephalic breeds and others prone to dystocia. Emergency cesareans follow hours of failing labour and carry considerably more risk.
Do OFA, PennHIP, or SV/WUSV x-rays require sedation?
It depends on the scheme. OFA hip and elbow films can often be taken without sedation using careful positioning. PennHIP and SV/WUSV both do require sedation, because each depends on precise positioning that cannot be achieved safely or accurately in an awake dog. We also perform thyroid, basic cardiac, patella, dentition, shoulder, spine and trachea clearances — the only ones we do not offer are those needing a specialist, meaning eyes and advanced cardiac.
How far do breeders travel to you?
Regularly from across Erie, Niagara, Genesee, Wyoming, Chautauqua, Cattaraugus, Orleans and Allegany counties, and routinely from Rochester, the Southern Tier, northwestern Pennsylvania and Southern Ontario. For most of Western New York we are considerably closer than the nearest university teaching hospital.
Still have a question? A two-minute call usually settles it.

